ESylwester Piaskowski, Malgorzata Szybka, Ewelina Stoczynska-Fidelus, Krystyna Hulas-Bigoszewska, Michał Bienkowski, Monika Witusik-Perkowska, Izabela Zawlik, Pawel P. Liberski, Piotr Rieske

Department of Tumour Biology, Medical University of Lodz, Poland

Abstract:

Developing of cell culture conditions allowing to preserve phenotype of glioma cells would be important. It was presented several times that original (in vivo presented) phenotype or even genotype of glioma cells is frequently not sustained in vitro. For example glioblastoma cells showing EGFR amplification cannot be easily analyzed in vitro. Intriguingly, we have recognized that similar problem applies to cells with IDH1 mutation. Glioblastoma cells presenting this alteration cannot be cultured in vitro under standard cell culture conditions. Reasons responsible for this bizarre behavior of glioblastoma cells should be recognized since it can help to define new therapeutic approach based on in vivo stimulating conditions responsible for in vitro tumor cells elimination. Lately, we have observed the first successful approaches to develop new conditions allowing to stabilize glioma cells showing IDH1 mutation.